2024 - Comparability of Five Alternative AAV9 Downstream Processes, from Ultracentrifugation to Chromatography

Improved analytical methods are helping improve the understanding of capsid heterogeneity in adeno-associated virus (AAV) therapeutic preparations. This understanding allows the development of manufacturing processes that produce AAV therapeutics with fewer non-functional capsids.
In this webinar, the speakers will present the results of a five-arm AAV9 comparability protocol that includes four alternative two-column downstream processes at 50-L scale and an ultracentrifugation (UC) process at 200-L scale. The UC process is the gold standard for functional capsid purification.
The expert speakers will outline simplified processes for both the capture and the empty/full (E/F) separation steps, including various combinations of cation exchange chromatography (CEX) and anion exchange chromatography (AEX) monoliths with different gradient types, as well as a process using an affinity resin and AEX monolith.
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Register for this webinar today to gain insights into the production of AAV therapeutics using enhanced analytical methods with their role in reducing capsid heterogeneity.
Keywords: Drug Development, Analytical Testing, Commercial Manufacturing, Bioanalytical Testing, AAV, Viral Vector, Viral Vector Manufacturing, Cell & Gene Therapies, AAV Manufacturing, CDMO/CMO, Quality, AAV Capsid