2024 - Boost CRISPR Editing Efficiency Using Optimized sgRNA and HDR Template Design

Clustered regularly interspaced short palindromic repeats (CRISPR) technology is now widely utilized in fields such as gene and cell therapy, animal and cell model development and genetic engineering. The rational design of single guide RNA (sgRNA) and homology-directed pair (HDR) template sequences is the most crucial step for the success of these experiments.
In this webinar, the expert speaker will share her extensive experience with CRISPR projects and discuss key insights for successful CRISPR experiments. Topics will include:
Designing highly specific sgRNAs with minimal off-target effects
Optimizing HDR templates to enhance knock-in efficiency
Practical demonstrations of sgRNA and HDR designs using GenScript’s tools for gene knockout, point mutation, small and large insertions and large fragment deletions
How to leverage GenScript’s free GenCRISPR sgRNA and HDR design tools to simplify the design process and improve editing efficiency
This webinar will thoroughly explore the intricacies of designing sgRNA and HDR templates for CRISPR gene editing, emphasizing high editing efficiency and minimizing off-target effects. The attendees will also learn the essential processes and skills required to achieve these objectives. The speaker will also discuss how to select the most appropriate sequences for different applications after considering specific parameters that influence the outcome.
Moreover, the session will include case studies covering various design scenarios, such as gene knockout, point mutations, small and large insertions and large fragment deletions, thus providing practical insights and examples. This comprehensive coverage will equip participants with the knowledge and tools necessary to execute precise and efficient gene editing techniques.
Register for this webinar today to gain expert insights into highly specific sgRNAs, enhancing knock-in efficiency with optimized HDR templates and optimizing CRISPR editing efficiency.
Keywords: Genetic Engineering, Drug Development, CRISPR, Clinical Research, CRO, Animal Models, Laboratory Technology, Cell and Gene Therapy, Cell & Gene Therapies, Basic Research, CGT