2024 - Exploring the Range of Electron Microscopes: Resolution, Size and Users

The introduction of direct electron detectors significantly improved the signal-to-noise ratio of electron micrographs, resulting in a marked enhancement in the quality of cryo-electron microscope (EM) reconstructions (now referred to as the “resolution revolution”).
This approach, along with advances in image processing and data collection, has pushed the resolution of single-particle cryo-EM reconstructions into the range where the electron source could become a limiting factor. This in turn has led to the reconsideration in cryo-EM of alternatives to the standard Schottky field emission gun (FEG), such as the cold FEG, owing to their superior coherence.
Despite the known benefits of the cold FEG over thermally assisted FEG, practical limitations prevented its use in cryo-EM until JEOL’s CryoARM microscope series was developed. Cold FEG was initially used on 300 kV cryomicroscopes to push the frontier of attainable resolution, ultimately resulting in cryo-EM reconstructions at “atomic resolution” where individual atoms are visible as separated spheres of density.
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Register for this webinar today to gain insights into cryo-electron microscopy and how the integration of cold FEG technology in JEOL’s CryoARM200 microscope is revolutionizing cryo-EM.
Keywords: Pharmaceutical, Drug Discovery, Biopharma, Biopharmaceutical, Diagnostics, Laboratory Technology, Pharma, Basic Research, Other Software, Electron Microscopy