2024 - Upstream Bioprocessing: DoE Optimization for CHO Cell Cultures

Chinese hamster ovary (CHO) cells are commonly used in bioprocessing to express desired proteins, including antibody, peptide and other protein-based therapeutics, at high titers. In upstream bioprocessing, process optimization and scale-up have presented challenges, and design of experiments (DoE) methodologies can alleviate these challenges and lay the foundation for a robust and scalable cell culture process.
Join this webinar to learn about upstream process development experiments conducted with MilliporeSigma across several CHOZN® cell lines at Culture Biosciences’ South San Francisco cell culture facility.
CHOZN® cell lines were evaluated with combinations of two batch media and three feed media variations in proprietary 250mL mammalian bioreactors for optimal combinations. The effect of each media combination on each cell line was evaluated for various quality attributes, including cell growth, lactate production and final immunoglobulin G (IgG) production. The proprietary purpose-built bioreactors were used to evaluate large-scale DoE experiments. Statistical analyses were performed to determine the effect of each component on the system. This work identified a significant increase in the final titer for two CHOZN® cell lines utilizing the same feed media. MilliporeSigma successfully validated these small-scale results at a 3 L scale, indicating the scalability of this mammalian cell culture process.
Join this webinar to learn how DoE optimization strategies set the foundation for robust upstream bioprocessing.
Keywords: Biomanufacturing, CDMO/CMO, Cell & Gene Therapies, AAV, Chinese Hamster Ovary, Bioprocess Development, Biologics Manufacturing, Protein Therapeutic, Antibody-Drug Conjugate, Monoclonal Antibodies, Antibodies, Process Development, Biosimilars, Cell Culture, Biologics, Antibody, Biosimilar, Drug Development, Gene Therapy