2023 - Best Practices and Benefits of qPCR Automation

The quantitative polymerase chain reaction (qPCR) has transformed the world of molecular biology methodology by enabling fast, high-throughput detection and quantification of DNA and RNA in real time. qPCR plays a vital role in addressing today’s healthcare questions, with applications ranging from gene expression analysis to mutagenesis studies. The revolutionary technique amplifies DNA in samples, enabling scientists to measure minute concentrations of analytes accurately. However, qPCR’s biggest strength also leads to its biggest challenge: any contamination in the sample, no matter how small, is also amplified, therefore increasing the need for qPCR automation.
Once contamination has been introduced into a qPCR experiment, it cannot be removed. It is, therefore, crucial to prevent contamination of the sample in the first place. While contamination can be effectively minimized during the liquid handling steps, manual processes in qPCR experiments inherently bring additional risks of introducing contamination and error, such as adding the wrong sample to the wrong well. Scientists can overcome this by qPCR automation processes such as liquid handling to minimize human intervention and therefore eliminate the risk of accidental contamination.
With over 50 years of experience, Gilson has the knowledge, expertise and equipment to support the scientific community in maintaining complete confidence in qPCR sample integrity. In this webinar, attendees will learn the fundamentals of qPCR automation and related techniques, as well as the many benefits that automation brings to the lab. Attendees will discover how labs can implement automation with practically no learning curve.
Join this webinar as the featured speaker will explore strategies for qPCR setup and related workflows using the PIPETMAX® and the best practices for workflow optimization, liquid handling and customization. They will also cover pre-written methods, popular software choices and training opportunities.