2023 - Enhancing AAV Full Capsid Enrichment with an Optimized AEX Platform: A Case Study

Successful separation of AAV empty and full capsids using anion exchange chromatography (AEX) requires consideration of both the purification platform and buffer composition to achieve high recovery and purity. Teknova and Sartorius BIA Separations have partnered together to demonstrate the critical nature of these two parameters — highly customized equilibration and elution buffer formulations plus AEX monolith technology — to establish a robust and scalable chromatographic profile. Using a DOE approach, the companies were able to achieve more than 85 percent full AAV capsid enrichment with 80-95 percent recoveries using CIMmultus QA for the polishing step.
Register for this webinar to learn about the critical parameters that affect full capsid enrichment in AAV purification using anion exchange chromatography.